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. 2020 Feb 7;8(1):73. doi: 10.3390/vaccines8010073

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ELISA reactivity of polyclonal rabbit serum raised to KLH-conjugated peptide or KLH-conjugated glycopeptide. Panels (A,C) coating antigen biotin-SML10 glycopeptide, panels (B,D) coating antigen biotin-SML10 peptide, Panel (E) coating antigen formalin killed C. difficile R20291 spores. Panel (A,B,E) serum IgG response and panels (C,D) serum IgA response. Legend: KLH-glycopeptide immune serum titration (black square), (KLH-peptide immune serum titration (circle), KLH-glycopeptide pre-immune serum titration (regular triangle), KLH-peptide pre-immune serum titration (inverted triangle).