Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 23;8(1):144. doi: 10.3390/vaccines8010144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Characterization of recombinant rabies viruses (rRABV) expressing OX40-ligand (OX40L) in vitro. (A) Construction diagram of LBNSE and LBNSE-OX40L. The vector pLBNSE was derived from the pseudogene-deleted SAD-B19 strain. The BsiWI and NheI sites were introduced between G and L genes. N, P, M, G, and L represented RABV nucleoprotein, phosphoprotein, matrix, glycoprotein, and polymerase genes, respectively. Murine OX40L was inserted into this LBNSE genome in place of the deleted pseudogene. Supernatants of the cells infected with different rRABVs at a MOI = 0.01 were collected at 1, 2, 3, 4, and 5 dpi to determine the titers of LBNSE and LBNSE-OX40L. Based on it, multiple-step growth curves of LBNSE and LBNSE-OX40L, respectively, infecting BSR cells (B) and NA cells (C) were developed. (D) The expression level of mouse OX40L in the infected cell supernatants was detected by ELISA kits. (E) Detection of cell viability of rRABV-infected BSR cells. Error bars represented the standard deviation (SD, n = 3).