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. 2020 Mar 23;8(1):144. doi: 10.3390/vaccines8010144

Figure 6.

Figure 6

Antibody responses and protection in mice immunized with LBNSE-OX40L. ICR mice (n = 10) were immunized via intramuscular (im) route with 100 μL volume containing 106 FFU of LBNSE or LBNSE-OX40L, or an equal volume of DMEM (as mock). Sera from vaccinated mice were collected weekly for nine weeks, and the virus-neutralizing antibodies (VNA) titers (A) and GMT (B) of sera were determined by using fluorescent antibody virus neutralization (FAVN). (C) The optical density (OD) values of total RABV G-specific immunoglobulin G (IgG), IgG1, IgG2a, IgG2b, IgG3, and immunoglobulin M (IgM) from immunized-mice sera were determined by ELISA. At 28 dpi, all the immunized mice (n = 15) were challenged with 50 LD50 of CVS-24 via ic route, and then they were carefully monitored daily for 21 days. The survival diagram was constructed according to the number of survivors in each group (D). Error bars represented the SE. The following notations were used to indicate significant differences between groups: *, p < 0.05; **, p < 0.01; ***, p < 0.001.