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In situ hybridization by RNAScope and H&E histology of brain sections. Stained tissue sections of V4020 and TC-83 brain tissue on day 2, 5, 7, 9, and 13 post IC inoculation. Brown staining in RNAScope images indicates the presence of viral RNA detected by an nsP3 in situ hybridization probe. Representative images at 50× magnification for RNAScope and 100× for H&E are shown. Arrows are used to indicate perivascular cuffing.
