Table 1.
Immune markers detected in the immunohistochemistry analysis.
| Antigen (Clone) | Specificity and Target Cells | Antigen Retreival Solution | Antibody Dilution | Reference |
|---|---|---|---|---|
| Bovine TNF-α (CC327) | Expressed in M1 macrophages. | 6 | 1:200 | [35] |
| Bovine IFN-γ (CC330) | Released by lymphocytes, macrophages and dendritic cells. Induces M1 macrophage polarization. | 6 | 1:100 | [36] |
| Human calprotectin (MAC387) | Expressed in activated, recently recruited macrophages among other cells. | 9 | 1:200 | [35] |
| Human CD163 (EDHu-1) | Expressed in M2 macrophages. | 6 | 1:300 | [35] |