Figure 1.

Statin dose–response curves via Alamar Blue dye reduction assay. Human breast cancer cell lines (SK-BR-3, HCC1419, MDA-MB-231, and MDA-MB-468) were treated with increasing concentrations of (A) Simvastatin or (B) Fluvastatin in the presence (short dash) or absence (long dash) of recombinant Th1 cytokines (Tumor Necrosis Factor-alpha, TNF-α and Interferon-gamma, IFN-γ, 10 ng/mL each) for 72 h. Alamar Blue dye was added and, following color change, the optical density of the dye in the culture supernatants was determined. Optical Density (OD) values of untreated controls (black) and cytokine only treatment (gray) are represented as horizontal lines.