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. 2020 Feb 6;8(1):72. doi: 10.3390/vaccines8010072

Figure 2.

Figure 2

Combination of Th1 cytokines and statin drugs potentiates metabolic suppression in breast cancer lines. SK-BR-3, HCC1419, MDA-MB-231, and MDA-MB-468 human breast cancer cell lines were cultured with no additives (No Tx), treated with recombinant Th1 cytokines (“Cyto” TNF-α and IFN-γ, 10 ng/mL each), statin drugs (Simvastatin or Fluvastatin, 1 µM MDA-MB-231; 10 µM remaining cell lines), or the combination of Th1 cytokines and a statin drug (“Statin + Cyto”). After 72h incubation, Alamar Blue dye was added and, following color change, optical density of culture supernatants was determined. Results displayed are from one representative experiment of at least four trials +/− Standard Error of the Mean (SEM). Letter designations represent Tukey’s Honest Significant Difference (HSD) comparisons: treatments with the same letter designation are not statistically different; when letter designations differ between treatments, the p-value is less than 0.05.