Table 3.

Primers used to confirm the presence of pathogenic DNA in tick samples.

Pathogen	Targeted Gene	Primer Name	Sequence (5′ → 3′)	Length (bp)	References
Rickettsia spp.	gltA	Rsfg877	GGGGGCCTGCTCACGGCGG	381	[67]
		Rsfg1258	ATTGCAAAAAGTACAGTGAACA
	ompB	Rc.rompB.4362p	GTCAGCGTTACTTCTTCGATGC	475	[68]
		Rc.rompB.4836n	CCGTACTCCATCTTAGCATCAG
		Rc.rompB.4496p	CCAATGGCAGGACTTAGCTACT	267
		Rc.rompB.4762n	AGGCTGGCTGATACACGGAGTAA
Anaplasma/Ehrlichia spp.	16S rRNA	EHR16SD	GGTACCYACAGAAGAAGTCC	345	[69]
		EHR16SR	TAGCACTCATCGTTTACAGC
Borrelia spp.	flaB	FlaB280F	GCAGTTCARTCAGGTAACGG	645	[70]
		FlaRL	GCAATCATAGCCATTGCAGATTGT
		FlaB737F	GCATCAACTGTRGTTGTAACATTAACAGG	407
		FlaLL	ACATATTCAGATGCAGACAGAGGT
Leishmania spp.	SSU rRNA	R221	GGTTCCTTTCCTGATTTACG	603	[71]
		R332	GGCCGGTAAAGGCCGAATAG
		R223	TCCATCGCAACCTCGGTT	358
		R333	AAAGCGGGCGCGGTGCTG