Figure 5.

Extracellular pH of the environment after 60 min (A) and 3 h (B) of interaction with A. fumigatus and C. lunata. Addition of serum alkalifies the media; significance of serum opsonization conditions were calculated compared to the untreated neutrophils with and without serum as well. Control: untreated hyphae or neutrophils; w supernatant: neutrophils were added directly to the germinated conidia; w/o supernatant: germinated conidia were washed with fresh modified Krebs-Ringer phosphate glucose (mKRPG) buffer before addition of neutrophils; serum opsonized: young hyphae were serum opsonized before addition of neutrophils. Error bars represent standard deviations calculated form three biological parallels. Multiple t-test was used in GraphPad Prism 7 software, FDR (Q = 10%), * p < 0.05.