Figure 2.

Physicochemical characteristics of the antigen loaded PLGA nanoparticles (microfluidics total flow rate (TFR) 10 mL/min, flow rate ratio (FRR) 1:1) and microparticles (double emulsion method) containing 10% (w/v) sucrose and 1% (w/v) L-leucine before freeze drying (FD) and after reconstitution of the dried powders: (A,D) Particle size, (B,E) polydispersity index (PDI) or SPAN and (C,F) Encapsulation efficiency (%) for nanoparticles and microparticles respectively. PDI is defined as the standard deviation of the particle size distribution divided by the mean particle diameter and gives an estimation of the uniformity of the particles. SPAN is also an indication of polydispersity for larger particles. All results represent the mean ± SD of at least three different batches.