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Antigen-specific humoral immune responses in (A) serum and (B) supernatants from lung lymphocytes after prime-pull immunisation (2 × s.c. CAF01:H56 and 1 × i.n. PLGA:H56). Serum samples from 6 individual mice in each vaccine group were isolated 2 weeks after the booster immunization. H56-specific serum production of immunoglobulin G (IgG) was measured by ELISA. ELISA OD450 values are represented as mean values ± SD, n = 6.
