FIG 3.

Growth phenotype of the WT D39 strain and ΔadcAII mutant. (A and B) Bacteria were inoculated at an OD₅₉₅ of 0.01 in THY (A) or CDM (B) supplemented with 33 μM Zn and incubated at 37°C for 8 h. Black circles, WT D39; white circles, ΔadcAII mutant. Two independent assays were performed using triplicate wells. Each point is the mean (SD) for the results of a representative experiment. (C) Mean (SD) WT D39 or ΔadcAII mutant CFU after culture in blood (1 ml inoculated with 1 × 10⁶ CFU) for 4 and 6 h. P values were calculated using unpaired Student’s t test. *, P < 0.05.