TABLE 1.
ΔadcAII mutant method of construction/source of DNA for the targeted deletion related to the capsule phenotype for multiple transformants
| DNA source for transformation | No. of clones analyzed |
Capsule phenotype |
||
|---|---|---|---|---|
| Absenta | Normal | Thick | ||
| PCR fragment adcAII::kana | 100 | 14 (1) | 32 | 44 |
| PCR fragment adcAII::cat | 100 | 45 (4) | 13 | 42 |
| Genomic DNA R6 ΔadcAII::cat1 1st | 4 | 0 | 0 | 4 |
| Genomic DNA R6 ΔadcAII::cat1 2nd | 4 | 1 | 0 | 3 |
| Genomic DNA R6 ΔadcAII::cat2 | 15 | 1 | 3 | 11 |
a
Numbers in parentheses are numbers of absent capsule strains sequenced all of which contained the Q308 stop codon mutation in cps2E.