TABLE 4.
Proportions of variants (identified by PCR analysis) for the SpnD39III (ST5556II) type I restriction-modification system for selected ΔadcAII mutant strains divided into those with thick and normal capsule thicknesses
| Phenotype | Strain | Proportion (%) of SpnD39III (ST5556II) variant: |
|||||
|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | ||
| Wild type | D39 | 2.2 | 0 | 67.2 | 15.6 | 0 | 15.0 |
| Thick capsule | Cl82 | 1.3 | 0 | 3.8 | 7.1 | 1.0 | 86.8 |
| Cl72 | 1.7 | 0 | 4.3 | 9.2 | 0 | 84.8 | |
| Cl10 | 1.3 | 0 | 4.0 | 8.8 | 0 | 85.9 | |
| Cl38 | 2.0 | 0 | 1.9 | 8.4 | 2.3 | 85.4 | |
| Cl3 2P | 1.0 | 0 | 2.5 | 6.4 | 1.6 | 88.6 | |
| Cl5 1P | 0.7 | 0 | 87.8 | 9.8 | 0 | 1.7 | |
| Cl3 1P | 1.7 | 0 | 84.8 | 10.3 | 0 | 3.3 | |
| Cl1 1G | 2.2 | 0 | 83.1 | 11.1 | 0 | 3.7 | |
| Cl7 1G | 0 | 0 | 7.7 | 92.3 | 0 | 0 | |
| AIIL | 3.8 | 0.6 | 74.9 | 17.8 | 0.0 | 2.8 | |
| Normal capsule | Cl88 | 0 | 0 | 6.0 | 92.7 | 0.00 | 1.3 |
| Cl28 | 0 | 0 | 6.7 | 93.3 | 0 | 0 | |
| Cl35 | 0 | 0 | 6.92 | 93.1 | 0 | 0 | |
| Cl6 | 0 | 0.7 | 5.71 | 93.6 | 0 | 0 | |
| Cl73 | 0 | 0 | 9.88 | 90.1 | 0 | 0 | |
| Cl20 | 0 | 0 | 9.93 | 90.1 | 0 | 0 | |
| Cl17 | 0 | 9.0 | 2.9 | 88.2 | 0 | 0 | |
| Cl1 | 0.4 | 1.7 | 16.4 | 80.8 | 0 | 0.6 | |
| Cl36 | 94.3 | 5.8 | 0 | 0 | 0 | 0 | |