TABLE 5.
Mutation construction, capsule phenotype, and (where available) cps2E gene genome sequence data for S. pneumoniae strains
| Strain/clone | Gene deletion | Antibiotic resistance |
Mutant construction | Capsule ratio/D39 | Capsule phenotype | Mutation in cps2E |
|---|---|---|---|---|---|---|
| D39 800 | None | 1 | Normal | None | ||
| D39 WT | None | 1 | Normal | None | ||
| ΔadcAIIL | ΔadcAII | Cm | New transformation | 3.7 | Thick | None |
| Cl10 | ΔadcAII | Kana | New transformation | 3 | Thick | E for K aaa 322 |
| Cl57 | ΔadcAII | Kana | New transformation | 0.5 | Unencapsulated | Stop codon aa 308 |
| Cl1 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 0.5 | Unencapsulated | Not sequenced |
| Cl1 1G | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3 | Thick | Not sequenced |
| Cl1 1G | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 2.9 | Thick | None |
| Cl2 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 0.5 | Unencapsulated | Not sequenced |
| Cl2 2P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 0.9 | Normal | Not sequenced |
| Cl3 1G | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.1 | Thick | Not sequenced |
| Cl3 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.2 | Thick | Not sequenced |
| Cl3 2P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.1 | Thick | None |
| Cl5 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.8 | Thick | None |
| Cl5 1G | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.7 | Thick | Not sequenced |
| Cl6 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 0.5 | Unencapsulated | Not sequenced |
| Cl6 2P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 0.5 | Unencapsulated | Stop codon aa 308 |
| Cl7 1P | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.5 | Thick | Not sequenced |
| Cl7 1G | ΔadcAII | Cm | Back-crossing with ΔadcAIIL | 3.1 | Thick | None |
| AIIcl 1 | ΔadcAII | Cm | New transformation | 1 | Normal | None |
| AIIcl 17 | ΔadcAII | Cm | New transformation | 1.1 | Normal | None |
| AIIcl 20 | ΔadcAII | Cm | New transformation | 1.1 | Normal | None |
| AIIcl 28 | ΔadcAII | Cm | New transformation | 1.15 | Normal | None |
| AIIcl 31 | ΔadcAII | Cm | New transformation | 0.5 | Unencapsulated | Stop codon aa 308 |
| AIIcl 35 | ΔadcAII | Cm | New transformation | 0.85 | Unencapsulated | Stop codon aa 308 |
| AIIcl 36 | ΔadcAII | Cm | New transformation | 1.05 | Normal | E for K aa 322 |
| AIIcl 38 | ΔadcAII | Cm | New transformation | 2.05 | Thick | E for K aa 322 |
| AIIcl 44 | ΔadcAII | Cm | New transformation | 0.5 | Unencapsulated | Stop codon aa 308 |
| AIIcl 72 | ΔadcAII | Cm | New transformation | 2.8 | Thick | E for K aa 322 |
| AIIcl 73 | ΔadcAII | Cm | New transformation | 1.15 | Normal | None |
| AIIcl 75 | ΔadcAII | Cm | New transformation | 1.2 | Normal | None |
| AIIcl 78 | ΔadcAII | Cm | New transformation | 1.8 | Thick | E for K aa 322 |
| AIIcl 82 | ΔadcAII | Cm | New transformation | 2.6 | Thick | E for K aa 322 |
| AIIcl 88 | ΔadcAII | Cm | New transformation | 1 | Normal | None |
| AII+Pcl4 | ΔadcAII + phtD | Cm | New transformation | 2.2 | Thick | Not sequenced |
a
aa, amino acid position.