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Substrate specificity of MccHHmi, HinTHmi, and HinTEco phosphoramidases. (A) HPLC elution profiles of products of incubation of εK-AMP with HinTEco, HinTHmi, or MccHHmi. (B) MALDI-TOF MS analysis of the hydrolysis reaction of the εK-AMP by HinTHmi protein. (C) Scheme of a HinT-mediated hydrolysis reaction of εK-AMP (36).
