FIG 3.

Plasmodium-parasitized blood protects against ECM and EAE independently from live parasites. (A) ECM development following infection with 10⁶ P. berghei (Pb) ANKA pRBC administered with or without sonicated extracts of P. berghei ANKA or P. berghei K173. (B, C) Proportions of CD69⁺ out of CD3⁺ T cells (B) and geometric mean fluorescence intensities of CD86 on CD11c⁺ cells (C) in the spleen at day 2 postinfection. Bars show the medians ± IQRs. (D) Experimental protocol. C57BL/6 mice were injected with P. berghei ANKA or P. berghei K173 pRBC sonicated extracts and immunized with MOG_35–55/CFA to induce EAE. (E) Clinical scores monitored up day 30 postimmunization. Dots show the medians ± IQRs. (F) ECM development following infection with 10⁶ P. berghei ANKA pRBC administered with or without sonicated extracts of 10⁶ P. yoelii 17X YM pRBC. (G, H) Proportions of CD69⁺ out of CD3⁺ T cells (G) and geometric mean fluorescence intensities of CD86 on CD11c⁺ cells (H) in the spleen at day 2 postinfection. Bars show the medians ± IQRs. (A, B, and C) Data representative of 3 independent experiments with N = 5 mice/group. (E) Data representative of 2 experiments. N = 6 mice/group. (F, G, and H) Data from 2 experiments with N = 5 mice per group.