FIG 8.

LDV alone protects against EAE by blocking the encephalitogenic CD4⁺ T cell response. C57BL/6 mice were injected with PBS or LDV 1 day before MOG_35–55/CFA immunization. Mononuclear cells from CNS and spinal cord harvested 15 days after MOG_35–55/CFA immunization were restimulated with 100 μg MOG peptide for 24 h. Production of IFN-γ, IL-17, and GM-CSF visualized by intracellular staining in CD4⁺ T cells from brain (A) and spinal cord (B). Numbers on the representative dot plots show the median percentages of cytokine-positive cells of CD44⁺ CD4⁺ T cells ± IQRs. Histograms show the absolute numbers. Data representative of 2 independent experiments with N = 7 mice/group.