TABLE 2.
β-Lactam resistance of B. ubonensis Bu278 complemented mutants
| Strain | Relevant genotype | MIC (μg/ml)a
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| MEM |
IMP |
CAZ |
AMX |
||||||
| −Ara | +Ara | −Ara | +Ara | −Ara | +Ara | −Ara | +Ara | ||
| Bu338 | Bu278::mini-Tn7-PBAD | ≥32 | ≥32 | ≥32 | ≥32 | 8 | 12 | ≥256 | ≥256 |
| Bu352 | Bu308::mini-Tn7-PBAD | 2 | 2 | 4 | 4 | 2 | 2 | ≥256 | ≥256 |
| Bu373 | Bu308::mini-Tn7-PBAD- nagZ+ |
≥32 | ≥32 | ≥32 | ≥32 | 4 | 16 | ≥256 | ≥256 |
| Bu356 | Bu296::mini-Tn7-PBAD | 1.5 | 2 | 6 | 6 | 1.5 | 2 | ≥256 | ≥256 |
| Bu375 | Bu296::mini-Tn7-PBAD-slt+ | ≥32 | ≥32 | ≥32 | ≥32 | 4 | 24 | ≥256 | ≥256 |
| Bu382 | Bu312::mini-Tn7 | 0.75 | NA | 0.19 | NA | 2 | NA | 192 | NA |
| Bu379 | Bu312::mini-Tn7-PpenB- penBBu278+ |
≥32 | NA | ≥32 | NA | 8 | NA | ≥256 | NA |
The MIC was determined using the Etest method, performed in triplicate on three separate days, and values are reported as the mode of the readings. −Ara, medium without l-arabinose; +Ara, medium with l-arabinose at a final concentration of 1%. NA, not applicable.