FIG 7.
The intracellular production of human azurocidin and cathepsin G proteins is toxic to A. fumigatus. (A) A. fumigatus wt and mutant strains Afazuro, AfcathG, and AfRBP7 harboring the human azurocidin, cathepsin G, and RBP7 genes, respectively, under the control of the tetON promoter. The cultures were grown for 24 h in the absence or the presence of doxycycline (DOX). (B) Biomass measurement of wt and A. fumigatus mutant strains Afazuro, AfcathG, and AfRBP7 with and without doxycycline. Data are representative of those from 3 independent experiments with 3 technical replicates. P values were determined by the Mann-Whitney test. ns, not significant; **, P < 0.01; ***, P < 0.001. (C) Detection of proteins produced in the A. fumigatus mutant strains. The bar plot shows the abundance level of the azurocidin protein for the Afazuro strain, the cathepsin G protein for the AfcathG strain, and RBP7 for the AfRBP7 strain, based on the intensity of the precursor ion. The data were generated from 3 analytical replicates.