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. 2020 Apr 15;13:37. doi: 10.1186/s13048-020-00639-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CTBP1-AS2 overexpression resulted in upregulation of PTEN. HEC-1 cells were transfected with CTBP1-AS2 expression vector or miR-216a mimic to analyze the interactions between CTBP1-AS2 and miR-216a. Overexpression of CTBP1-AS2 and miR-216a was confirmed at 48 h post-transfection (a). The effects of CTBP1-AS2 and miR-216a overexpression on expression of each other were analyzed by qPCR (b). QPCR and western blot were performed to analyze the effects of CTBP1-AS2 and miR-216a overexpression on the expression of PTEN at mRNA (c) and protein (d) levels, respectively. Mean values of 3 biological replicates were calculated and compared by unpaired t test.*,p < 0.05