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. 2020 Apr 14;17:115. doi: 10.1186/s12974-020-01800-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Validation of ACSA-2 astrocyte cell enrichment from brain tissue. a Generalized workflow for ACSA-2 magnetic bead enrichment of astrocytes from the injured brain parenchyma. Digested cell suspensions were labeled with the ACSA-2 magnetic bead before being placed in the magnetic column for removal of non-specific cells, which were collected into a tube as the ACSA-2^neg. fraction. Removal of the column from the magnetic stand allowed the flow-through of the retained ACSA-2 astrocytes to be collected as the ACSA-2^pos. fraction. RNA from both fractions was harvested to examine gene expression endpoints. b Gene expression analyses using putative markers of five neural tissue subsets: astrocyte (Aldh1l1), neuronal (Dlg4), oligodendroglial (Klk6), microglial (Tmem119), and endothelial (Nostrin). These data demonstrate a significant induction of putative astrocyte signature (orange bar), with little signature of other cell populations (blue bars). Log₂ fold change is a ratio of ACSA-2^pos to ACSA-2^neg. TLDA, Taqman low density array cards