Table 2.

Multivariate analysis between clinical signs and gut microbial classes

Dry eye signs	Class	p†	Comparison with the literature
DEQ5	Methanobacteriaceae	< 0.01	↑ in RA and ulcerative Colitis [18]
	Bifidobacteriaceae	< 0.01
	Eggerthellaceae	0.012	↓ in myasthenia gravis [19]
	Flavobacteriaceae	< 0.01	↓ in myasthenia gravis [19]
	Eubacteriaceae	<0.01	↑ in type 1 diabetes [20]
	Peptococcaceae	< 0.01	↓ in SLE [21]
	Ruminococcaceae	< 0.01	↓ in IBD and psoriasis [22]
	Erysipelotrichaceae	< 0.01
	Leptotrichiaceae	< 0.01
	Synergistaceae	< 0.01
MMPWorse	Porphyromonadaceae	0.042	↑ in ankylosing spondylitis [23]
	Acidaminococcaceae	< 0.01
OSDI	Rikenellaceae	0.046	↑ in ankylosing spondylitis [23]
Schirmer	Elusimicrobiaceae	< 0.01
	Carnobacteriaceae	< 0.01
	Clostridiaceae	< 0.01	↑ in SLE
	Clostridia Family XI	< 0.01	↑ in RA and IBD-arthritis [24]
	Clostridia Family XIII	< 0.01
	Fusobacteriaceae	< 0.01	↑ IBD [21]
	Leptotrichiaceae	< 0.01
	Akkermansiaceae	< 0.01
Stainworse	Methanomassiliicoccaceae	0.028
	Pasteurellaceae	< 0.01	↑ in myasthenia gravis [19]

DEQ5 dry eye questionnaire 5, OSDI ocular surface disease Index, RA Rheumatoid arthritis, IBD inflammatory bowel disease, SLE systemic Lupus Erythematosus

^†Multivariable analysis considered the effects of demographics (age, gender, race, ethnicity). Dry eye signs not listed in table (e.g. tear break up time) did not exhibit significant associations with gut microbial classes, when considering demographics

*For all dry eye signs, value from more severely affected eye used in the analysis