Table 1.
Principal oligonucleotide primers used in this study
| Primer | Primer sequence (5′ → 3′) | Amplicon size (bp) | Parameters of PCR |
|---|---|---|---|
| P1 | TGCTGTGCTCGTCTAAT | 3531 | (95 °C, 5 min) + {(95 °C, 30 s) + (60 °C, 30 s) + (72 °C, 3 min 35 s)} × 34 + (72 °C, 5 min) |
| P2 | AATGCCTGGCGTGTTT | ||
| P3 | TGGCTATCACAACAAGG | 1190 | (95 °C, 5 min) + {(95 °C, 30 s) + (60.5 °C, 30 s) + (72 °C, 1 min 15 s)} × 29 + (72 °C, 5 min) |
| P4 | TTAGAACTAGGGCAGGA | ||
| P5 | CGCTTAACTAAGTGCAAGAA | 1008 | (95 °C, 5 min) + {(95 °C, 30 s) + (61.2 °C, 30 s) + (72 °C, 1 min 5 s)} × 29 + (72 °C, 5 min) |
| P6 | GCTACGCTGAGAACCATC |