Fig. 5.

Silencing of miR-543 promotes RA-FLS proliferation and inflammation and inhibits apoptosis, which could be reversed by silencing of PVT1 or SCUBE2 in RA. Synovial cells were treated with sh-SCUBE2 or sh-PVT1 in the presence of miR-543 inhibitor or miR-543 mimic alone. a The proliferation of cells in each group detected by EdU staining (× 100). b Cell cycle changes in each group measured by flow cytometry. c The apoptosis rate of cells in each group detected by flow cytometry. d The expression of IL-1β in cell culture media determined by ELISA. e Expression of Bax, Bcl2, PCNA, and Ki67 in cells tested by western blot analysis. *p < 0.05 vs. synovial cells treated with mimic NC; ^#p < 0.05 vs. synovial cells treated with inhibitor NC; ^&p < 0.05 vs. synovial cells treated with miR-543 inhibitor + sh-NC. All data were measurement data and presented as mean ± standard derivation. The ANOVA was used for the comparison among multi-groups, of which comparing between two groups adopted Tukey’s post hoc test; the experimental data was obtained from the average of 3 independent experiments