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. 2020 Apr 14;21:241. doi: 10.1186/s12891-020-03267-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Attenuation of fluorescent signal by blood. a: Relative fluorescent signal in the injured knee, ΔFMT, depicted as function of joint diameter increase, Δdiameter, 24 h after induced joint bleed shows low ΔFMT at high Δdiameter. b: Excitation, fluorescence, and reconstructed image of a haemophilic mouse with a Δdiameter of 1.64 mm (a) and 0.49 mm (b) at 24 h. Colour bar shows intensity of signal. Note the attenuated signal in mouse a. c: Fluorescent signals measured with FMT after adding 500 pmol AngioSense750 to a 1.5 mL Eppendorf tube containing either 100% PBS; 50% v/v PBS and whole blood; or 100% whole blood (mean +/− standard deviation), as shown in (d)