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. 2020 Apr 15;53:13. doi: 10.1186/s40659-020-00282-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Growth characteristics and chemosensitivity analysis. a Dose–response curves of the ascites-derived gallbladder cancer cell lines treated with chemotherapeutic agents. Cells were incubated for 72 h with each drug as single agent before cell viability was assessed via MTS assay. Data are representative of three independent experiments with three technical replicates (mean ± SD) b Mean doubling times (hours) and half maximal inhibitory concentration (IC₅₀) values of cytotoxic drugs of ascites-derived clones and commercial established GBC cell lines