Fig. 4.

Effects of PERK inhibition on BIX-01294-induced ROS generation and ROS-induced apoptosis of KG1 LSC-like cells. a, Intracellular ROS generation, measured by flow cytometry, in cells treated with BIX-01294 (10 μmol/L) in the absence or presence of GSK2606414 (20 μmol/L) for 16 h. b, ROS generation after cotreatment of cells with BIX-01294 (10 μmol/L) and GSK260641 (20 μmol/L) with/without preincubation with NAC (1 mmol/L) for 1 h. c, Apoptosis levels, determined by flow cytometry, after cotreatment of cells with BIX-01294 (10 μmol/L) and GSK2606414 (20 μmol/L) for 48 h, with/without preincubation with NAC (1 mmol/L) for 1 h. d, Protein expression, analyzed by western blotting with the indicated antibodies, after cotreatment of cells with BIX-01294 (10 μmol/L) and GSK2606414 (20 μmol/L) for 48 h, with/without preincubation with NAC (1 mmol/L) for 1 h