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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Endocr Relat Cancer. 2018 Apr 25;25(7):735–746. doi: 10.1530/ERC-17-0496

Figure 1.

Figure 1

The amino terminus of GREB1 isoforms interact with ERα. (A) Endogenous GREB1 was immunoprecipitated from MCF7 whole-cell lysate and purified complexes were subjected to immunoblot analysis with GREB1 and ERα antibodies. (B) 3xFLAG-tagged fragments of GREB1a were generated according to the schematic. (C) Plasmids expressing the individual fragments were co-transfected with ERα into HEK-293AD cells and immunoprecipitated from whole-cell lysate using anti-FLAG magnetic beads. Immunoblot analysis of input and FLAG IP lysates was performed with FLAG and ERα antibodies. (D) 3xFLAG-tagged GREB1a, GREB1b, GREB1c or empty vector (EV) was co-transfected with ERα into HEK-293AD cells. Immunoprecipitation with anti-FLAG magnetic beads was followed by immunoblot analysis of input and FLAG IP lysates with FLAG and ERα antibodies.