Table 2.

Analysis of Molecular Variance (AMOVA) of epiloci, overall and for each type, under both directions of treatment's shift, for the groups of the same individuals of Ranunculus kuepferi. 2x: Diploid, 4x: Tetraploid.

Epiloci	Overall				Externally-methylated				Internally-methylated				Non-methylated
Shift's direction	Warm to Cold		Cold to Warm		Warm to Cold		Cold to Warm		Warm to Cold		Cold to Warm		Warm to Cold		Cold to Warm
Ploidy level	2×	4×	2×	4×	2×	4×	2×	4×	2×	4×	2×	4×	2×	4×	2×	4×
AMOVA global
Variation among groups (%)	33.38	40.08	31.61	36.01	10.26	22.78	19.28	15.3	48.22	55.93	31.61	57.8	45	57.18	49.54	51.68
Variation within groups (%)	66.62	59.92	68.39	63.99	89.74	77.22	80.72	84.7	51.78	44.07	68.39	42.2	55	42.82	50.46	48.32
ΦST	0.33	0.4	0.32	0.36	0.1	0.23	0.19	0.15	0.48	0.56	0.32	0.58	0.45	0.57	0.5	0.52
P-value	<0.001	<0.001	<0.001	< 0.001	<0.001	<0.001	<0.001	<0.001	<0.001	< 0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001
AMOVA locus-by-locus
Significantly differentiated loci	30	41	24	38	7	19	8	18	16	10	8	12	6	12	6	9
Significantly differentiated loci (%)	30.93	38.68	25	34.86	12.96	30.64	15.38	27.69	66.66	50	33.33	60	31.58	50	30	37.5