TABLE 17-1.
List of Selected Antigen Markers, Sources, Tissue Controls, and Uses for Selected Antibodies Used in Dogs and Cats
| Antigen | Species* | Clone/Catalog # | Vendor | Tissue Control | Use |
|---|---|---|---|---|---|
| Actin muscle | Dog | HHF35 | Dako | Skeletal muscle/heart | Muscle neoplasms |
| Actin sarcomeric | Dog | Alpha-Sr-1 | Dako | Skeletal muscle/heart | Striated muscle tumors |
| Actin smooth muscle | Dog | 1A4 | Dako | Stomach/Intestine | Smooth muscle tumors |
| Adenovirus (blend) | Dog | 20/11 and 2/6 | Chemicon | Infected tissue | Infection |
| Amylin (IAPP) | Cat, Dog | R10/99 | AbD Serotec | Pancreas | Pancreatic islet amyloid |
| Bcl-2 oncoprotein | Cat only | NCL-bcl-2 | Novocastra | Lymphoid tissue | Lymphoid tumors |
| B-lymphocyte antigen (BLA.36) | Cat, Dog | A27-42 | Dako | Lymph node, spleen | B-cell, histiocytic tumors |
| CD1c (ICC) | Dog only | CA13.9H11 | UCD | Lymphoid tissue | Dendritic cell tumors |
| CD3 (ICC) | Dog only | CA17.2A12 | AbD Serotec | Lymph node, spleen | T-cell lymphoma |
| CD3 epsilon (IHC) | Cat, Dog | CD3-12 | AbD Serotec | Lymph node, spleen | T-cell lymphoma |
| CD10 (CALLA antigen) | Dog | 56C6 | Vector | Kidney, | Renal, stromal tumors |
| CD11d (IHC) | Dog only | CA18.3C6 | UCD | Spleen | Lymphoid, histiocytic tumors |
| CD11d (ICC) | Cat, Dog | CA16.3D3 | UCD | Spleen | Lymphoid, histiocytic tumors |
| CD18 | Cat only | FE3.9F2 | UCD | Spleen | Leukocytic tumors |
| CD18 | Dog only | CA16.3C10 | UCD | Spleen, lymph node | Leukocytic tumors |
| CD20 | Cat, Dog | RB-9013 | LabVision | Spleen, lymph node | B-cell tumors |
| CD31 | Dog | JC/70A | Dako | Skin, other | Vascular endothelial and megakaryocytic tumors |
| CD45 | Dog only | CA12.10C12 | UCD | Spleen, lymph node | Leukocytic tumors |
| CD45RA | Dog only | CA21.4B3 | UCD | Lymphoid tissue | Lymphoid tumors |
| CD79a | Cat, Dog | HM57 | Dako | Lymph node, spleen | B-cell lymphoma |
| CD117 (c-Kit protein) | Dog | A4502 | Dako | Mast cell tumor | Mast cell tumor |
| Calcitonin | Cat, Dog | A0576 | Dako | Thyroid | C-cell tumors |
| Calponin | Dog | CALP, h-CP | Dako, Sigma | Small intestine, stomach | Smooth muscle and myoepithelial tumors |
| Calretinin | Cat, Dog | 18-0211 | Zymed | Kidney | Renal tubules, nerve tissue, adrenocortical tumors, mesothelioma |
| Canine distemper virus | Dog | CDV-NP | VMRD | Infected tissue | Infection |
| Carcinoembryonic antigen | Dog | A0115 | Dako | Intestine | Epithelial tumors |
| Caspase-3 | Dog | CASP3ACTabr | Research Diagnostics | Lymph node | Apoptotic cells |
| Chromogranins A + B | Dog | PRO11422 | Research Diagnostics | Pancreas | Neuroendocrine marker |
| Coronavirus | Cat, Dog | FIPV3-70 | CMI | Infected tissue | Infection |
| COX-1 | Dog | 160108 | Cayman Chemical | Normal urinary bladder | Normal urothelium, endothelium |
| COX-2 | Cat, Dog | PG 27 B | Oxford Biomedical | Transitional cell carcinoma | Carcinomas |
| Cytokeratin 5 | Dog | XM26 | Vector | Mammary gland, skin | Myoepithelium, basal cells |
| Cytokeratin 7 | Dog | OV-TL 12/30 | Dako | Skin, urinary bladder | Glandular epithelium |
| Cytokeratins 8/18 | Dog | 5D3 | Novocastra | Liver, stomach | Glandular epithelium |
| Cytokeratins AE1-AE3 | Cat, Dog | AE1 and AE3 | Dako | Skin | General epithelium marker |
| Cytokeratins Pan | Dog | MNF116 | Dako | Glandular/squamous epithelium | General epithelium marker |
| Cytokeratins HMW | Dog | 34βE12 | Dako | Skin | Squamous epithelium, mesothelium, hepatocytes |
| Desmin | Dog | D33 | Dako | Skin, stomach, intestine | Muscle tumors |
| E-Cadherin | Dog | 36 | BD Transduction | Skin | Langerhan's cells, epithelium, histiocytoma, meningiomas |
| Estrogen receptor alpha | Cat, Dog | CC4-5 | Novocastra | Uterus | Estrogen receptor tumors |
| Factor VIII–related antigen | Dog | A0082 | Dako | Skin, other | Vascular endothelial and megakaryocytic tumors |
| Feline calicivirus | Cat | S1-9 | CMI | Infected tissue | Infection |
| Feline herpesvirus 1 | Cat | FHV5 | CMI | Infected tissue | Infection |
| Feline leukemia virus | Cat | C11D8-2C1 | CMI | Infected tissue | Infection |
| Glial fibillary acidic protein | Dog | Z0334 | Dako | Brain | Neural (glial) tumors |
| Glucagon | Cat, Dog | A0565 | Dako | Pancreas | Glucagon-producing tumors |
| Glut 1 | Dog | A3536 | Dako | Peripheral nerve | Peripheral nerves, stromal cells, kidney |
| Hepatocyte marker-1 (Hep Par 1) | Dog | OCH1E5 | Dako | Liver | Hepatocellular tumors |
| Ig kappa chains | Dog | A0191 | Dako | Lymph node | Plasmacytomas |
| Ig lambda chains | Dog | A0193 | Dako | Lymph node | Plasmacytomas |
| Immunoglobulin M | Cat, Dog | CM7 | CMI | Lymph node | Lymphoid tumors |
| Inhibin-alpha | Dog | R1 | Serotec | Testicle, Sertoli cell tumor | Sex cord–stromal and adrenal cortical tumors |
| Insulin | Dog | Z006 | Zymed | Pancreas | Insulin-producing tumors |
| Ki-67 | Dog | 7B11 | Zymed | Lymphoma | Cell proliferation marker |
| Laminin | Cat, Dog | Z0097 | Dako | Skin/Kidney | Perivascular wall tumors and basement membrane |
| Leptospira | Dog | − | NVSL | Infected tissue | Infection |
| Lysozyme | Dog | A0099 | Dako | Liver, spleen | Histiocytes |
| Melan A | Cat, Dog | A103 | Dako | Melanoma | Melanomas, steroidproducing tumors |
| MHC II | Dog | TAL.1B5 | Dako | Histiocytoma, LN | Antigen presenting cells, lymphocytes |
| Microphthalmia transcription factor | Dog | C5 | abcam | Melanoma | Melanomas |
| MUM 1 protein | Dog | MUM1p | Dako | Plasmacytoma | Plasmacytomas, myelomas, some B-cell tumors |
| Myeloid/histiocytic antigen | Dog | MAC 387 | Dako | Spleen, liver | Macrophages, myeloid cells |
| Myoglobin | Dog | A324 | Dako | Skeletal muscle, heart | Skeletal muscle tumors |
| Neospora caninum | Dog | 210-70-NC | VMRD | Infected tissue | Infection |
| Nerve growth factor receptor | Dog | NGFR5 | LabVision | Nerve | Nerves |
| Neurofilament-2 | Dog | SMI-31 | Covance | Brain | Neuron neurofilaments |
| Neuron specific enolase | Dog | BBS/NC/VI-H14 | Dako | Pancreas | Neuroendocrine marker |
| OCT3/4 | Dog | C-10 | Santa Cruz | Mast cell tumor | Germ cell and mast cell tumors |
| p63 | Dog | 4A4 | Santa Cruz | Skin, mammary gland | Myoepithelium, basal cells |
| Papilloma virus | Dog | B0580 | Dako | Infected tissue | Infection |
| Parathyroid hormone | Dog only | M7070 | Dako | Parathyroid | Normal and neoplastic parathyroid |
| Parvovirus | Dog | A3B10 | VMRD | Infected tissue | Infection |
| Progesterone receptor | Dog | SP21 | LabVision | Uterus | Progesterone receptor tumors |
| Proliferating Cell Nuclear Antigen | Dog | PC10 | Dako | Lymphoma, lymph node | Proliferation marker |
| Protein Gene Product 9.5 | Cat, Dog | Z5116 | Dako | Adrenal gland | Neuroendocrine marker |
| S-100 protein | Cat, Dog | Z0311 | Dako | Nerve, brain | Neural marker, neuroendocrine tumors |
| Somatostatin | Dog | A0566 | Dako | Pancreas | Pancreatic islet tumors, some carcinoids |
| Synaptophysin | Dog | SP11 | LabVision | Pancreas | Neuroendocrine marker |
| Thyroglobulin | Cat, Dog | 492020 | ShandonImmunon | Thyroid | Thyroglobulin-producing tumors |
| Thyroid transcription factor-1 | Dog | 8G7/G3/1 | Dako | Lung, thyroid | Lung and thyroid neoplasms |
| Toxoplasma gondii | Cat | MAB802 | Chemicon | Infected tissue | Infection |
| Tryptase | Dog only | AA1 | Dako | Mast cell tumor | Mast cell tumors |
| Uroplakin III | Dog only | AU1 | Res. Diagnostics | Urinary bladder | Urothelial neoplasms |
| Vimentin | Dog | SP20 | LabVision | Skin, stomach | Mesenchymal tumor marker |
CMI, Custom Monoclonals International; HMW, high molecular weight; NVSL, National Veterinary Services Laboratories (Ames, IA); UCD, University of California-Davis (P. Moore)
*
Known species reactivity is listed; Dog or Cat only – indicates both species were tested but only one is reactive