TABLE 17-7.
Antibody Panels for Characterization of Canine and Feline Leukocytes by Flow Cytometry
| Cell Type | Antigen | Clone(s) | Species Antibody Produced Against |
|---|---|---|---|
| Dog Panel | |||
| Dendritic cells/monocytes | CD1c | CA13.9H11 | Dog |
| T cells | CD3 | CA17.2A12 | Dog |
| T cell subset/neutrophils | CD4 | YKIX302.9/CA13.1E4 | Dog |
| T cells | CD5 | YKIX322.3 | Dog |
| T cell subset | CD8α | YCATE55.9/CA9.JD3 | Dog |
| Macrophages, some T-cells | CD11d | CA16.3D3 | Dog |
| Monocytes/Neutrophils* | CD14 | TUK4/UCHM1 | Human |
| B cells | CD21 | CA2.1D6/LB21 | Dog/human |
| Precursors | CD34 | 1H6 | Dog |
| B cells | CD79a | HM57 | Human |
| All leukocytes | CD45 | YKIX716.13/CA12.10C12 | Dog |
| Cat Panel | |||
| T cells | CD3ɛ | CD3-12 | Human |
| T cell subset | CD4 | vpg39 | Cat |
| T cells | CD5 | FE1.1B11 | Cat |
| T cell subset | CD8α/β | vpg9 | Cat |
| Monocytes | CD14 | TUK4* | Cat |
| B cells | CD21 | CA2.1D6/LB21 | Dog/human |
| B cells | CD79a | HM57 | Human |
*
TUK4 does not appear to stain neutrophils, but UCHM1 does.