Figure 2.

Autophagy negatively regulates ciliogenesis in HK2 cells. Serum-deprived cells were treated with autophagy modulators: Bafilomycin A1 (BafA1), Rapamycin (RAPA), Tat-Beclin1 peptide (Tat-B1). In the graphs below the influence of autophagy on cilia length is represented as fold change. In all graphs, white bars correspond to untreated (NT) cells, while black bars represent cells treated with autophagy modulators. Bars = 10 μm. Hoechst (blue) was used to stain nuclei. Data are presented as the mean ± SEM. To evaluate significance t-test (graph in B, ^***P-value < 0.005) was used. n = number of replicates.