Figure 5.

Loss of VPS39 decreases the satellite pool of OFD1. (A) OFD1 centrosomal dynamics in serum deprivation. OFD1 (green) decreases in the pericentriolar region up to 24 h after serum deprivation and increases during prolonged starvation. (B) OFD1 (green) levels decrease around the centrosome in VPS39-silenced (siVPS39) cells (left panels and black bar in the graph) Bars = 10 μm. Hoechst (blue) was used to stain nuclei. Data are presented as the mean ± SEM. t-test was used to evaluate significance ^***P-value < 0.005. n = number of replicates.