Figure 6.

VPS39 influences ciliogenesis in vivo. (A) Expression of human (h) VPS39 mRNA transiently injected in medaka fish embryos (1/2 cell stage). Fish were collected at stage36 and 9-(hatching), 11- and 13-days post-injection and mRNA levels were analyzed by real-time PCR. Expression levels of hVPS39 were maintained up to day 9 (hatching stage) in injected fish (black bars) compared to controls (white bars). (B) IF with acetylated tubulin (red) revealed that hVPS39 overexpression (middle panels) results in fewer ciliated renal tubule cells compared to controls (upper panels). When fish are treated with CQ an increased number of ciliated cells was detected. DAPI was used to stain nuclei. One tail t-test was used to evaluate significance. Data are presented as the mean ± SEM. ^**P-value < 0.01.