Table 1.
Primers for quantitative polymerase chain reaction
| Gene | Sequence | Length(bp) |
|---|---|---|
| β-actin | Forward: GTGACGTTGACATCCGTAAAGA | 22 |
| Reverse: GTAACAGTCCGCCTAGAAGCAC | 22 | |
| ApoE | Forward: CAGACCTTTGGGAAGAGCGG | 20 |
| Reverse: CCAGTCTGCGGAGGGAAAAA | 20 | |
| FAS | Forward: GGCCCCTCTGTTAATTGGCT | 20 |
| Reverse: GGATCTCAGGGTTGGGGTTG | 20 | |
| I-FAPB | Forward: GGACTGGACCTCTGCTTTCC | 20 |
| Reverse: CACCGAGCTCAAACACAACA | 20 | |
| ACC1 | Forward: ATGTGGCCTGGGTAGATCCT | 20 |
| Reverse: CGGACAAGGTAAGCCCCAAT | 20 | |
| PPARγ | Forward: GACGTTTGTGGCTGGTCAAG | 20 |
| Reverse: CAGTGGGGAGAGAGGACAGA | 20 | |
| SCD1 | Forward: TGGAGACGGGAGTCACAAGA | 20 |
| Reverse: ACACCCCGATAGCAATATCCAG | 20 | |
| ZO-1 | Forward: CTGGTGAAGTCTCGGAAAAATG | 22 |
| Reverse: CATCTCTTGCTGCCAAACTATC | 22 | |
| occludin | Forward: TGCTTCATCGCTTCCTTAGTAA | 22 |
| Reverse: GGGTTCACTCCCATTATGTACA | 22 | |
| Bacteroides | Forward: TGACAGTGAGAGATTTGCTGCGTA | 24 |
| Reverse: TCAGCCGACATTTCCTCTTCCGA | 23 | |
| Bifidobacteria | Forward: GATTCTGGCTCAGGATGAACGC | 22 |
| Reverse: CTGATAGGACGCGACCCCAT | 20 | |
| Lactobacillus | Forward: AGCAGTAGGGAATCTTCCA | 19 |
| Reverse: CACCGCTACACATGGAG | 17 | |
| Enterobacter cloacaeRuminococcus | Forward: TATCCGTCGCCCATACAGA | 19 |
| Reverse: TTGATCCGATCCACCACCForward: GCACAAGCAGTGGAGTReverse: CTTCCTCCGTTTTGTCAA | 181618 | |
| 16S rRNA | Forward: CCTACGGGAGGCAGCAG | 17 |
| Reverse: ATTACCGCGGCTGCTGG | 17 |