Figure 1.

rmhTNF suppressed the survival of human CRC cells. HCT116 (a), SW480 (b), HCT8 (c) and LOVO (d) cells were incubated with different concentrations of rmhTNF for 24, 48, or 72 h, respectively. The cell viability was detected by the MTT assay. *P < 0.05, **P < 0.01, ***P < 0.001 vs. untreated groups (from 24, 48, and 72 h, respectively) (Student’s t test). The IC₅₀ values of HCT116 (e), SW480 (f), HCT8 (g), and LOVO (h) cells were calculated by the GraphPad Prism 5 software. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test). Results represented mean ± SD of three replicates. IC₅₀: half inhibitory concentrations. (A color version of this figure is available in the online journal.)