Fig. 3.

Biological effects of EBP50 and IRSp53 downregulation by siRNAs (NEM157-i line). (A) Western blot validation of downregulation by siRNAs. Percent downregulation is indicated on graphs. (B) Cell proliferation assays 96 hours after transfection measured by sulforhodamine B assay. (C) Cell death was measured by FACS with annexinV–fluorescein isothiocyanate/PI staining 72 hours after transfection. (D) Migration 48 hours after transfection was measured using wound healing assay. Cells were incubated 6 hours after injury. (E) Invasion was measured 48 hours after transfection by wound healing assay coated with Matrigel. Cells were incubated 48 hours after injury. Representative photos of one experiment used for quantification are shown. All graphs were normalized to siCtrl.