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. 2020 Feb 6;8(1):70. doi: 10.3390/vaccines8010070

Figure 9.

Figure 9

MVA-B ΔA40R-rev downregulated the mRNA levels of type I IFN, proinflammatory cytokines, and chemokines. Human THP-1 macrophages were mock infected or infected with MVA-WT, MVA-B, MVA-B ΔA40R, or MVA-B ΔA40R-rev at 5 PFU/cell. At 3 and 6 h.p.i., RNA was extracted, and IFN-β, IFIT1, IFIT2, MDA-5, TNF-α, MIP-1α, RANTES, RIG-I, HPRT, and VACV E3L mRNA levels were analyzed by RT-qPCR. Results are expressed as the ratio of the gene of interest to VACV E3L mRNA levels. A.U.: arbitrary units. p values indicate significant response differences between MVA-B, MVA-B ΔA40R, and MVA-B ΔA40R-rev at the same hour (**, p < 0.005; ***, p < 0.001). Data are means ± standard deviations of triplicate samples from one experiment and are representative of two independent experiments.