(A to C) CVB3/28 decay in TBS with 10% FBS (A), in TBS with 10% FBS, 1.8 mM CaCl2, and 0.8 mM MgCl2 (B), and in DMEM with 10% FBS and 5 mM EDTA (C). The kapp values were 0.125 h−1 (SE = 0.014 h−1) (A), 0.085 h−1 (SE = 0.013 h−1) (B), and 0.127 h−1 (SE = 0.016 h−1) (C) and were not statistically different.