FIG 6.
SDS-PAGE of Affi-Gel blue pools that were not reduced (A), reduced (B), and (C) Western blot probed with anti-BSA. Mr indicates the molecular masses (in kilodaltons) of the marker proteins in lane 1 of all three panels. (A and B) Lanes 2 contain proteins not bound to the Affi-Gel blue, and lanes 3 and 4 contain proteins in the two pools (one broad protein peak) that eluted from the column. (C) The blot shows the combined eluate pools analyzed that were not reduced (lane 2) and reduced (lane 3).