FIG 9.

Coomassie blue-stained gel of nonreduced bovine serum albumin (2.6 μg/lane) (A) purified from FBS following chromatography on PBE 94; (B) high-molecular-weight fraction obtained by gel filtration of commercial essentially fatty acid-free bovine serum albumin (Sigma A-0281); (C) monomeric BSA fraction obtained by gel filtration chromatography of the Sigma BSA. Protein size markers are in the left lane, with their molecular masses indicated to the left in kilodaltons. The band in lane A near 100 kDa corresponds to the BSA dimer as shown in Fig. 6.