TABLE 1.

CVB3 decay experiments

Strain and medium (Figure)	FBS (%)	BSA (μM)	No. of decay expts	kapp (h−1)	SE (h−1)
CVB3/28 in DMEM and DMEM-10 (Fig. 1)
DMEM	0	0	2	0.041	0.003
DMEM	8.9	24	2	0.111	0.006
Varied concns of FBS in DMEM (Fig. 2)
DMEM (same as shown in Fig. 1A)	0	0	2	0.041	0.003
DMEM	0.1	0.27	2	0.047	0.003
DMEM	0.5	1.4	2	0.051	0.004
DMEM	1	2.7	2	0.076	0.005
DMEM	5	14	2	0.113	0.003
DMEM	8.9	24	1	0.113	0.008
DMEM	10	27	1	0.11	0.006
DMEM	20	54	2	0.118	0.004
CVB3/28 L1092V and CVB3/28 A3180T (Fig. 4)
CVB3/28 L1092V
DMEM	0	0	2	0.03	0.004
DMEM	10	27	2	0.03	0.004
CVB3/28 L3180T
DMEM	0	0	2	0.038	0.003
DMEM	10	27	2	0.057	0.004
PBE 94 pool A BSA and Sigma BSA in PBS (Fig. 8)
PBS, PBE 94 pool A	0	4	2	0.198	0.013
PBS, Sigma BSA	0	4	2	0.27	0.019
PBS, FBS, BSA (from Affi-Gel blue) (Fig. 10)
PBS	0	0	10	0.038	0.002
PBS	10	27	6	0.276	0.009
PBS	0	3.8	3	0.212	0.007
Varied concns of FBS in PBSa (Fig. 11)
PBS	0	0	1	0.043	0.005
PBS	0.1	0.27	1	0.043	0.007
PBS	1	2.7	1	0.294	0.03
PBS	5	14	1	0.35	0.028
PBS	10	27	1	0.289	0.031
PBS	20	54	1	0.252	0.018
TBS-10, Ca-Mg, EDTA (Fig. 13)
TBS	10	27	1	0.125	0.014
TBS + 1.8 mM Ca + 0.8 mM Mg	10	27	1	0.085	0.013
DMEM + 5 mM EDTA	10	27	1	0.127	0.016
FBS effect is reversibleb (Fig. 14)
PBS	0	0	2	0.038	0.004
PBS	20	54	2	0.038	0.004
PBS + 1 mM EDTA	20	54	1	0.045	0.005
FBS potentiates sCARc (Fig. 15)
DMEM, 100 nM sCAR	0		2	0.558	0.097
DMEM, 250 nM sCAR	0		2	1.41	0.094
DMEM, 500 nM sCAR	0		2	1.543	0.107
DMEM, 1,000 nM sCAR	0		2	1.512	0.13
DMEM, 100 nM sCAR	9.69/10	26/27	2	1.526	0.09
DMEM, 250 nM sCAR	9.62/10	26/27	2	1.536	0.101
DMEM, 500 nM sCAR	9.51/10	26/27	2	1.743	0.12
DMEM, 1,000 nM sCAR	9.28/10	25/27	2	1.699	0.129

^aData are fit for two virus populations; see the details in Fig. 11.

^bTwo hours at 37°C and then centrifuged through sucrose and assay stability.

^cFBS was diluted by added sCAR in the first of two experiments.