TABLE 1.

Hydrogen-deuterium exchange collection statistics

Criterion	Connector only	Connector + PNTD
HDX reaction details	200 mM NaCl, 20 mM HEPES, 1 mM TCEP, 0.02% NaN3, pHread 7.00, 20°C
HDX time course (min)	0, 1, 2, 3, 4, 5
HDX control samples	Fully labeled control (9-day exchange)
Back exchange (%) (mean/SD)	19/12
No. of peptides	50
Sequence coverage (%)	90.5
Avg peptide length/redundancy (amino acid residues)	14.5, 2.8
No. of technical replicates	3
Repeatability (%D) (avg SD)	1.2	1.3
Significance criterion for HDX (no. of D)	0.961 (99% confidence)