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. 2020 Feb 28;94(6):e01175-19. doi: 10.1128/JVI.01175-19

FIG 6.

FIG 6

DR sequences also control production of DENV2 and ZIKV sfRNAs. (A) Secondary structure of ZIKV 3′-UTR. The different RNA secondary structures and CS sequences are indicated. (B) Northern blot of total RNA extracted from C6/36 cells infected with recombinant ZIKV with RCS3 or CS3 deletions. (C) Growth kinetics of recombinant ZIKVs with individual RCS3 and CS3 deletions in C6/36 cells. RCS3-del, CS3-del, and WT virus titers showed no significant differences at different time points. n.s., no statistically significant differences. (D) Secondary structure of DENV2 3′-UTR. The different RNA secondary structures and CS sequences are indicated. (E) Northern blot analysis of total RNA extracted from C6/36 cells infected with recombinant DENV2 containing RCS3 and RCS3/CS3 deletions. (F) Growth curves of recombinant DENV2 viruses in C6/36 cells. RCS3-del, RCS3/CS3-del, and WT virus titers showed no significant differences at different time points. n.s., no statistically significant differences.