FIG 9.

Three-dimensional RNA structure model of sfRNA. The first 350 nt of 3′-UTR from SLI to CS3 was used for three-dimensional (3D) RNA structure model analysis performed with the RNAComposer Web server with standard settings. The color of each structure matches the color of the labeled box. RCS3 is depicted as black in a square box. For WT and P4-1c, a complete pairing of RCS3 stem resulted in very tight tertiary folding, whereas for P4-1a or P4-1b mutant RNA, impaired base pairing of RCS3 stem resulted in loose tertiary folding.