FIG 16.

Dual-luciferase reporter assay for EBOV vncRNA function. 769-P (A and C) or EpoNi/22.1 (B and D) cells were transfected with the indicated pmirGLO dual-luciferase reporter constructs and were either infected with wt rEBOV (A and B) or subsequently transfected with a synthetic siRNA homologous to the EBOV GP vncRNA at a final concentration of 50 nM (C and D). For each assay, FLuc values were normalized to the corresponding RLuc values to generate a ratio; the data are expressed as the mean FLuc/RLuc ratio ± standard deviation proportional to that for the empty-vector control construct. All assays were performed in biological quadruplicate (n = 4). One-way analysis of variance followed by a Dunnett multiple-comparison posttest was used to assess statistical significance, and all comparisons were made to the empty-vector-transfected group. Multiplicity-adjusted P values are indicated by letters above the bars. (A) A, P < 0.0001; B, P < 0.0001; C, P < 0.0001; (B) A, P = 0.0065; (C) A, P < 0.0001; B, P = 0.0145; C, P = 0.0064; (D) A, P < 0.0001; B, P = 0.0017; C, P = 0.0018. Data are representative of two independent experiments.