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. 2020 Feb 28;94(6):e01441-19. doi: 10.1128/JVI.01441-19

FIG 8.

FIG 8

Filovirus vncRNAs exhibit cell line-dependent differential abundances. For each virus and time point, the abundance of each vncRNA relative to vRNA abundance from the EpoNi/22.1 and RO6E/J libraries was compared to the abundance in HepG2 cells. The normalization procedure is described under “Bioinformatics” in Materials and Methods. All statistical comparisons were made against HepG2 libraries by using two-way analysis of variance followed by Dunnett’s multiple-comparison posttest, except in the case of panel A, where Sidak’s multiple-comparison posttest was used. Letters above bars indicate significance; P values are given in the legend for each panel. (A) wt rEBOV–eGFP at 12 hpi. A, P = 0.0067. Note that RO6E/J cells were omitted from this analysis. (B) wt rEBOV–eGFP at 24 hpi. A, P ≤ 0.0001; B, P = 0.0172. (C) rEBOV VP35 R312A–eGFP at 12 hpi. A, P = 0.0448. (D) rEBOV VP35 R312A–eGFP at 24 hpi. A, P ≤ 0.0001; B, P ≤ 0.0001; C, P = 0.0012; D, P ≤ 0.0001. (E) rMARV wt–eGFP at 12 hpi. A, P ≤ 0.0001. (F) rMARV wt–eGFP at 24 hpi. A, P = 0.0245; B, P ≤ 0.0001; C, P = 0.0102; D, P ≤ 0.0001. (G) rMARV VP35 R301A–eGFP at 12 hpi. A, P = 0.0026. (H) rMARV VP35 R301A–eGFP at 24 hpi. A, P = 0.0472; B, P = 0.0341; C, P ≤ 0.0001; D, P ≤ 0.0001; E, P ≤ 0.0001. For all panels, the means for three biological replicate sequencing libraries for each group ± standard deviations are plotted.