FIG 3.

Syndecan-1 loss during HSV-1 infection is not due to transcriptional changes in the syndecan protein. (A) mRNA expression shows no significant change in syndecan-1 transcripts at either 24 or 36 hpi. HCE cells were infected with KOS-WT at an MOI of 0.1, and mRNA was extracted from cells at the indicated time points. Results are shown as fold change of infected over uninfected cells at each time point, normalized to GAPDH. (B, left) Western blotting (WB) shows no significant change in protein expression of syndecan-1 after 24 and 36 hpi. HCE cells were infected with KOS-WT at an MOI of 0.1. (B, right) Densitometry quantification of syndecan-1 normalized to GAPDH. The fold change of infected over uninfected cells is shown for each time point.