FIG 5.

MMP-3 and MMP-7 are upregulated upon HPSE overexpression or HSV-1 infection. (A) HPSE overexpression increases production of MMP-3 and MMP-7 transcripts. Cells were transfected with HPSE vector, and mRNA was collected 48 h posttransfection. Quantitative PCR was performed with the primers MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9. (B) When cells were infected with HSV-1 for 24 h, the increase in MMP profile was similar to that of HPSE-overexpressed cells with significant increases in MMP-3 and MMP-7. (C) Flow cytometry analysis shows progressively increased expression of MMP-3 on the surface of HCE cells when infected with HSV-1. The gray histogram shows the isotype antibody; black indicates uninfected, and red shows infected cell populations. Cells were analyzed at 24 hpi. (D) Flow cytometry analysis shows progressively increased expression of MMP-7 on the surface of HCE cells when infected with HSV-1. The gray histogram shows the isotype antibody; black and red indicate uninfected and infected cell populations, respectively. Cells were analyzed at 24 hpi. (E) Immunofluorescence imaging shows a similar increase in the expression of MMP-3 on the surface of infected cells. Red represents surface MMP-3, and blue shows 4′,6-diamidino-2-phenylindole (DAPI). Images were taken at 0 and 36 hpi. (F) Immunofluorescence imaging shows a similar increase in the expression of MMP-7 on the surface of infected cells. Red represents surface MMP-7, and blue shows DAPI. Images were taken at 0 and 36 hpi. (G) Quantification of the immunofluorescence results demonstrates a significant increase in surface MMP3 expression at 36 hpi. (H) Quantification of the immunofluorescence results demonstrates a significant increase in surface MMP7 expression at 36 hpi.